When using Unity iPhone 1.7, the behavior of this feature is different than when using other versions of Unity in the following way: When enabled,
ONLY the project folder will be scanned and sprites/controls in the current scene will not be used when creating the atlas(es). When using this feature in Unity iPhone, make sure all of the sprites in your scenes are tied to a prefab in the project folder. When doing this, make sure to only make modifications directly to the prefabs themselves and not to the instances in any particular scene as doing so will cause the scene instance to "override" the prefab, and it will not be updated with the new UVs, etc.